Posters

Posters

A map with Poster ID locations will be available in each poster room.

Complete abstracts are available in the SMLMS Attendee Handbook.

P01
The hunting of the synthase: single molecule tracking of the bacterial synthase PBP2B to elucidate the dynamics of the bacterial division complex.

Tregidgo N., Cesbron Y., and Holden S.

Centre for Bacterial Cell Biology, Institute of Cell and Molecular Biology, Newcastle University, Newcastle upon Tyne NE2 4AX, UK.

P02
Near isotropic super-resolution imaging of the bacterial cytoskeleton

Cesbron Y., Jukes C., Tregidgo N., Holden S.

Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, UK

P03
Nanorulers and Nanobeads for 2D and 3D Resolution Analysis

Scheible M.B.1, Forthmann C.1, Tinnefeld P.2, and Schmied J.J.1

1 – GATTAquant GmbH, Braunschweig Germany
2 – TU Braunschweig, Institute for Physical Chemistry, Braunschweig Germany

P04
Combining density-based artifact-free and Bayesian cluster analysis tools for studying the characteristics of membrane-protein organization

Kutz S.1, de Gouvello A.2, Ewers H.1.

1 – Institute of Chemistry and Biochemistry, Freie Universität Berlin, Germany
2 – ENSTA ParisTech, Université de Paris-Saclay, France

P05
Quantitate analysis of cytoskeletal nanoarchitecture at the T cell immunological synapse

Peters R., Griffié J., Williamson D.J., and Owen D.M.

Department of Physics and Randall division of cell and molecular biophysics, King’s College London, London.

P06
Localizing ALS associated protein FUS within synaptic compartment using super-resolution microscopy techniques

Deshpande D.K.1, Demestre M.2, Bockers T.2, and Jens Michaelis J.1

1 – Department of Biophysics, University of Ulm, Germany
2 – Department of Anatomy and Cell-biology, University of Ulm, Germany

P07
The nanoscale organisation of HIV cell surface receptors CD4 and CCR5

Jacobs C.1, Pereira P.1,2, Lawrence S.1, Marsh M.1, and Henriques R.1,2

1 – MRC Laboratory for Molecular Cell Biology, University College London, London WC1E 6BT, UK
2 – Cell Developmental Biology Department, University College London, London WC1E 6BT, UK .

P08
Super-resolution imaging at the cell-nanopattern interface

Reynolds P.M., Cutiongco M.F., Barbour E., and Gadegaard N.

Division of Biomedical Engineering, School of Engineering, Rankine Building, University of Glasgow, G12 8LT, UK

P09
Poxvirus nanoscale ultrastructure visualized by single-molecule localization microscopy

Albrecht D., Robert Gray R., Henriques R., and Mercer J.

MRC-Laboratory for Molecular Cell Biology. University College London, Gower Street, London, WC1E 6BT, United Kingdom

P10
Clutch engagement, actin dynamics and nanoscale integrin adhesion clustering in high speed T cell migration

Shannon M.1, Pineau J.1, Griffie J.1, Almada P.2, Williamson D.J.1, Owen D.M.1, Cornish G.H.1, and Cope A.1

1 – King’s College London, UK,
2 – University College London, UK

P11
Interpreting the complexity of folded protein transport by single-molecule microscopy

Hickman S.J.1,2, Alcock F.1, Kapanidis A.2, and Berks B.1

1 – Department of Biochemistry, University of Oxford, South Parks Road, Oxford, UK
2 – Department of Physics, University of Oxford, South Parks Road, Oxford, UK

P12
Cholesterol- and neuronal activity-dependent dopamine transporter nanodomains revealed by super-resolution microscopy

Lycas M.D.1, Rahbek-Clemmensen T.2, Erlendsson S.1, Eriksen J.1,2, Apuschkin M.1, Vilhardt F.3, Jørgensen T.N.1, Hansen F.H.1, and Gether U.1,2.

1 – Molecular Neuropharmacology and Genetics Laboratory, Center for Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen DK
2 – Lundbeck Foundation Center for Biomembranes in Nanomedicine, Center for Neuroscience, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen DK
3 – Department of Cellular and Molecular Medicine, Panum Institute, University of Copenhagen, Copenhagen, DK

P13
Visualizing lamin networks and their interactions with nuclear pore complexes by SMLM

Schoen I.1,2, Aires L.1, Ries J.3, and Vogel V.1

1 – ETH Zurich, Laboratory of Applied Mechanobiology, 8093 Zurich, Switzerland
2 – Royal College of Surgeons in Ireland (RCSI), Laboratory of MechanoVascular Biology and Microscopy, Dublin, Ireland
3 – EMBL, Laboratory of Superresolution Microscopy for Structural Cell Biology, Heidelberg, Germany

P14
Nano-scale organisation of synaptic proteins in the plasma membrane of presynaptic boutons induced on micropatterned host substrates.

Nosov G.1,2, Trahe J.1,2, Krishnan S.2, and Klingauf J.2

1 – University of Muenster, Muenster, Germany
2 – Institute of Medical Physics and Biophysics, Muenster, Germany.

P15
Universal modular subsystem for 3D localization based imaging and tracking using engineered point spread functions

Agrawal A.1, Gaumer S.1, Heiser K.1, Piestun R.1,2, and Kimerling L.1

1 – Double Helix LLC, Boulder, Colorado
2 – Department of Electrical, Computer and Energy Engineering, U. of Colorado, Boulder

P16
Fluorescence and superresolution imaging of alpha-synuclein aggregation

Thevathasan J. and Ries J.

European Molecular Biology Laboratory, Cell Biology & Biophysics Unit, Heidelberg, Germany

P17
Temporal reconstruction of yeast endocytosis by single molecule localization microscopy

Hoess P., Mund M., Deschamps J., and Ries J.

European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Heidelberg Germany

P18
Quantitative Single Molecule Localization Microscopy with Bruker’s Vutara SRX Analysis Software

Ebeling C.G.

Bruker Fluorescence Microscopy, Middleton Wisconsin USA

P19
Reasons for photon count underestimation of Gaussian fits to single molecule data

Thorsen R., Stallinga S., and Rieger B.

Department of Imaging Physics Delft University of Technology Lorentzweg 1, 2628 CJ Delft, The Netherlands

P20
Learning Single Molecule Localisation

Steuer H. and Kapanidis A.N.

Clarendon Laboratory, University of Oxford, Oxford, United Kingdom

P21
Combined atomic force microscopy and fluorescence super-resolution microscopy

Hirvonen L.M., Marsh R., Staszowska A.D., Jones G.E., Cox S.

Randall Division of Cell and Molecular Biophysics, King’s College London, UK

P22
Quantifying protein copy numbers in super-resolution using a DNA origami platform

Cella Zanacchi F.1,2, Manzo C.1, Sandoval Álvarez Á.1, Derr N.D.3, García-Parajo M.1,4 and Lakadamyali M.1

1 – ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology, 08860 Castelldefels (Barcelona), Spain
2 – Italian Institute of Technology, Via Morego 30, 16163 Genova, Italy
3 – Smith College, 44 College Lane, Northampton, MA 01063, USA 4 ICREA, Pg. Lluís Companys 23, 08010 Barcelona, Spain

P23
Real-time live-cell super-resolution imaging with SRRF-Stream

Culley S.1, Browne M.2, Gribben H.2, Catney M.2, Coates C.2, Wilde G.2, Henriques R.1,3

1 – MRC Laboratory for Molecular Cell Biology, University College London, London, UK
2 – Andor Technology, Belfast, Northern Ireland
3 – Department of Cell and Developmental Biology, University College London, London, UK

P24
Open-source, Lego® based live-to-fixed single-molecule localization microscopy bridges the dynamics versus resolution gap

Almada P. and Henriques R.

MRC Laboratory for Molecular Cell Biology, London, UK

P25
Characterization and Visualization of Ceramides with Molecular Resolution

Schlegel J.1, Walter T.2, Becam J.3, Burgert A.1, Schubert-Unkmeir A.3, Seibel J.2, and Sauer M.1

1 – Department of Biotechnology and Biophysics, Julius-Maximilian University Wuerzburg, Biocenter – Am Hubland, 97074 Wuerzburg, Germany
2 – Institute for Organic Chemistry, Julius-Maximilian University Wuerzburg, Am Hubland C1, Wuerzburg, Germany
3 – Institute of Hygiene and Microbiology, Julius-Maximilian University Wuerzburg, Wuerzburg, Germany

P26
Plasmon-selective hot-electron reactions enables nanoscale control of the surface chemistry on nano-structures

Simoncelli S., Li .Y, Cortés E., and Maier S.A.

The Blackett Laboratory, Department of Physics, Imperial College London, London SW7 2AZ, United Kingdom

P27
Structural insights into nanocompartmentalized stimuli responsive microgels by superresolution fluorescence microscopy

Oppermann A., Gelissen A.P.H., Richtering W., and Wöll D.

Institute of Physical Chemistry, RWTH Aachen University, Aaachen Germany

P28
Application of localization microscopy for single-molecule analysis in polymer materials

Aoki H.

J-PARC Center, Japan Atomic Energy Agency

P29
PSF-shaping with deformable mirror improves in-cell 3D localization microscopy

Aristov A., Lelandais B., Lelek M., and Zimmer C.

Imaging and modelling unit, Institut Pasteur, 25-28 Rue Docteur Roux, 75015 Paris

P30
Blue and infrared light induced photoconversion of green-to-red fluorescent proteins as a new approach in single-molecule localization microscopy

Turkowyd B.1, Balinovic A.1, VirantD.1, Gölz Carnero H.G.1, Caldana F.1, Endesfelder M.2, Bourgeois D.3, Endesfelder U.1

1 – Department of Systems and Synthetic Microbiology, Max Planck Institute for Terrestrial Microbiology & LOEWE Center for Synthetic Microbiology (SYNMIKRO), Karl-von-Frisch-Str. 16, 35043 Marburg, Germany
2 – Institut für Assyriologie und Hethitologie, Ludwig-Maximilians-Universität München, GeschwisterScholl-Platz 1, 80539 München, Germany
3 – Institut de Biologie Structurale, CNRS, Université Grenoble Alpes, CEA, IBS, 38044 Grenoble, France

 

P31
Super-Resolution Imaging of Native Cellulose Nanostructure

Babi M., Fatona A., and Moran-Mirabal J.

Department of Chemistry and Chemical Biology, McMaster University, Hamilton, Canada

P32
New ultra-low distortion imaging instrumentation offers dual-technique possibilities.

Cadby A., Olivier N., and Barnett S.

P33
Photoswitching of green mEos2 by intense 561-nm light perturbs efficient green-to-red photoconversion in localization microscopy

Thédié D., Berardozzi R., Adam V., and Bourgeois D.

Institut de Biologie Structurale, Université de Grenoble Alpes, CEA, CNRS, F-38044 Grenoble, France

P34
Deciphering the Receptome by dSTORM

Eiring P., Götz R., Kuhlemann A., and Sauer M.

University of Wuerzburg, Department of Biotechnology and Biophysics

P35

Nanoscale organisation of apical polarity proteins in Drosophila epithelia

Edo Dzafic, Nick Lowe, George Sirinakis, Edward Alleger, Daniel St Johnston

Wellcome Trust/Cancer Research UK Gurdon Institute, Cambridge, UK