Conference program
Day 1 – Wednesday 27 August 2025
Day 2 – Thursday 28 August 2025
Day 3 – Friday 29 August 2025
Time (Europe / Bonn)
Event
11:00 – 13:00
Registration
12:45 – 13:00
WelcomeOfficial words of welcome & practical information
13:00 – 15:00
Session 1chair Kristin Grußmayer
13:00 – 13:30
Invited speaker 1 – Michelle Frei: “Chemigenetic tools for live-cell fluorescence microscopy”
13:30 – 14:00
Invited speaker 2 – Sang-Hee Shim: “Caged RESI: Boosting RESI Performance via Reduction and Photoactivation”
14:00 – 14:20
Abstract talk 1 – Eduard M. Unterauer: “Towards spatial proteomics at single protein resolution”
14:20 – 14:40
Abstract talk 2 – Dominik Wöll: “Localization-based super-resolution and single molecule microscopy studies on soft nanoparticles”
14:40 – 14:50
Technology showcase 1 – Steffen Frey, NanoTag Biotechnologies GmbH: “Rethink Immunofluorescence: SmartSecondaries for One-Step IF and Species-Independent Multiplexing”
15:00 – 15:30
Coffee Break
15:30 – 17:00
Session 2chair Christoph Spahn
15:30 – 16:00
Invited speaker 3 – Johan Elf: “Combining single molecule detection with optical pooled screening”
16:00 – 16:20
Abstract talk 3 – Laura Jaekel: “EVE is an open modular data analysis software for event-based localization microscopy”
16:20 – 16:40
Abstract talk 4 – Tanja Menche: “Quantitative mapping of MHC I clustering using single-molecule localization microscopy”
17:00 – 18:00
Public Keynote 1 Xavier Darzaq: “Biochemistry: from cold to dark rooms – Measuring molecular interactions in live cells using Single Molecule Imaging”
18:00 – 19:00
Fingerfood Dinner
19:00 – 21:00
Poster Session 1 with beer & fingerfood
Time (Europe / Bonn)
Event
08:50 – 09:00
Welcome Info for the day
09:00 – 11:10
Session 3chair Ignacio Izeddin
09:00 – 09:30
Invited speaker 4 – Maja Klevanski: “Automated multiplexed dSTORM imaging of the spatial co‑distribution of proteins and glycans”
09:30 – 10:00
Invited speaker 5 – Jarno Mäkela: “Single-molecule dynamics of heat shock proteins in live Escherichia coli”
10:00 – 10:20
Abstract talk 5 – Johannes Hohlbein: “The Escherichia coli replication initiator DnaA is titrated on the chromosome”
10:20 – 10:40
Abstract talk 6 – Qingru Li: “Cryo-4Pi single-molecule localization microscopy”
10:40 – 11:00
Abstract talk 7 – Johannes Stein: “Cryosectioning-enhanced super-resolution microscopy for single-protein imaging across cells and tissues”
11:00 – 11:10
Technology showcase 2 – Mehdi Madi, Abbelight: “Abbelight Nanoscopy Solution – Unlocking Spatial Proteomics at Single-Protein Resolution”
11:10 – 11:40
Coffee Break
11:45 – 12:45
Public Keynote 2 Zan Luthey-Schulten: “4D simulations of a growing and dividing minimal cell: An integration of experiments, theory, and simulations”
12:45 – 13:30
Fingerfood Lunch
13:30 – 15:30
Poster Session 2 with drinks & fingerfood
15:30 – 17:30
Session 4chair Bernd Rieger
15:30 – 16:00
Invited speaker 6 – Steffen Sahl: “Intramolecular MINFLUX analysis”
16:00 – 16:30
Invited speaker 7 – Siegfried Musser: “Overlapping Nuclear Import and Export Paths Unveiled by Two-Color MINFLUX”
16:30 – 16:50
Abstract talk 8 – Paul Welzl: “Super-resolved imaging of native mRNA particle ultrastructure”
16:50 – 17:10
Abstract talk 9 – Jonas Ries: “Evaluating MINFLUX experimental performance in silico”
17:10 – 17:30
Abstract talk 10 – Ran Huo: “High-throughput single molecule microscopy with adaptable spatial resolution using exchangeable oligonucleotide labels”
18:00 – 22:00
Conference Dinner in the botanical garden
18:00 – 18:45
Guided tours through the garden (details TBC)
18:45 – 19:00
Reception in the greenhouse
19:00 – 22:00
Flying Dinner
Time (Europe / Bonn)
Event
08:50 – 09:00
Welcome Info for the day
09:00 – 10:50
Session 5chair Susan Cox
09:00 – 09:30
Invited speaker 8 – Anna-Karin Gustavsson: “Single-objective light sheet microscopy with microfluidics for improved 3D single-molecule super-resolution imaging”
09:30 – 10:00
Invited speaker 9 – Estibaliz Gomez de Marisca: “Harnessing AI towards the limits of live microscopy imaging”
10:00 – 10:20
Abstract talk 11 – Rémi Galland: “In depth volumetric SMLM imaging using Adaptive Optics and single-objective light-sheet microscopy at the entire cell scale and beyond”
10:20 – 10:40
Abstract talk 12 – Sebastian Bauer: “SMLMFlow: Enhancing Structural Resolution in SMLM with GNNs and Flow Matching”
10:40 – 10:50
Technology showcase 3 – Mathias Pasche-Drews, Teledyne Scientific Imaging Photometrics: “Scientific Cameras for SMLMS & How Teledyne Photometrics can support you with your science!”
10:50 – 11:30
Coffee Break
11:30 – 13:30
Session 6chair Koen Martens
11:30 – 11:50
Abstract talk 13 – Yoav Shechtman: “One-click image reconstruction in single-molecule localization microscopy via deep learning”
11:50 – 12:10
Abstract talk 14 – Hannah Kim: “O-SNAP: A comprehensive pipeline for spatial profiling of chromatin architecture”
12:10 – 12:40
Invited speaker 10 – Mahipal Ganji: “Multiplexed DNA-PAINT for Super-Resolution Mapping of Nuclear Architecture”
12:40 – 13:10
Invited speaker 11 – Helge Ewers: “Controlled release of single molecules by optogenetic uncaging”
13:15 – 13:30
Conclusion
13:30 – 14:30
Lunch bags
