Program

Scientific Program

“Single Molecule Localization Microscopy Symposium 2018”
SMLMS2018

August 27th – 29th, 2018
Harnack Haus of the Max-Planck-Society
Berlin
Germany

Monday, August 27th

HourDescription
11:00 – 13:30Arrival & registration
(posters can be placed upon arrival)
11:00Guided tour around the historical Campus Dahlem
13:30Introductory remarks
Helge Ewers / Mike Heilemann
13:40 – 15:00Biological Applications I
Chair: Aleksandra Radenovic, EPFL
   13:40Greg Giannone (CNRS, France)
Deciphering the spatiotemporal and mechanical regulation of actin and integrin regulators at the nanoscale
   14:05Melike Lakadamyali (University of Pennsylvania, USA)
Shedding new light on intracellular transport regulation with super-resolution microscopy
   14:30Marie Lena Harwardt (Goethe University Frankfurt, Germany)
Diffusion dynamics, activation, and crossinteraction of receptor tyrosine kinases studied by single-particle tracking
   14:45Charlotte Kaplan (University of California, Berkeley, USA)
Mechanoregulated actin network organization and function in endocytosis
15:00 – 17:30Poster Session & Coffee
17:30Keynote lecture: Luke Lavis, HHMI, USA
Designing brighter dyes for single-molecule imaging
Chair: Helge Ewers / Mike Heilemann
19:00Welcome reception & dinner

Tuesday, August 28th

HourDescription
9:00 – 10:20Probes and labeling technologies I
Chair: Luke Lavis
   9:00Ralf Jungmann (LMU, Germany)
Super-Resolution Microscopy with DNA Molecules
   9:25Michelle Peckham (Leeds University, United Kingdom)
Affimers, super-resolution imaging and analysis of protein organisation in muscle Z-discs
   9:50Richard Wombacher (Heidelberg University, Germany)
Fluorogenic Probes for Bioorthogonal Chemistry – Application in Live-Cell SMLM Imaging
   10:05Tobias Lutz (University of Exeter, United Kingdom)
Alternatives for tuning of the binding event rate in DNA-PAINT
10:20Coffee break in poster area
11:10 – 12:30Data Analysis
Chair: Melike Lakadamyali
   11:10Susan Cox (King’s College London)
Fast super-resolution imaging without artefacts
   11:35Suliana Manley (EPFL, Switzerland)
3D particle reconstruction and autonomous acquisitions in SMLM
   12:00Wei Ouyang (Institut Pasteur Paris, France)
Reducing artefacts in SMLM with deep neural networks and data sharing platform for the community
   12:15Johannes Hohlbein (Wageningen University & Research, Netherlands)
Monitoring target search of CRISPR-Cas in vivo
12:30Lunch break
14:00 – 15:20Immune Cells
Chair: Suliana Manley, EPFL
   14:00Dylan Owen (King’s College London, United Kingdom)
Post-processing analytics for describing protein nanoscale spatio-temporal organisation
   14:25Marcus Taylor (Max Planck Institute for Infection Biology Berlin, Germany)
DNA based T cell receptors: new tools for single molecule imaging of T cell activation
   14:50Sabrina Simoncelli (King’s College London, United Kingdom)
Multicolor super-resolution fluorescence imaging of protein interactions during T-cell activation
   15:05Andreas Arnold (TU Wien, Austria)
The TCR is randomly distributed in the plasma membrane of resting T cells
15:20 – 17:30Poster Session & Coffee
17:30Keynote lecture: Katharina Gaus, UNSW, Australia
T cell receptor clustering – a mechanism of signal transduction
Chair: Helge Ewers / Mike Heilemann
19:00Conference Dinner

Wednesday, August 29th

HourDescription
9:00 – 10:20Optical Technologies
Chair: Katharina Gaus
   9:00Sandrine Leveque-Fort (Université Paris-Sud, France)
Merging axial single molecule localization strategies for robust multicolor 3D imaging
   9:25Jonas Ries (EMBL, Germany)
Nuclear pores as universal reference standards for quantitative microscopy
   9:50Kristin Grussmayer (STI/IBI-EPFL Lausanne, Switzerland)
Multi-Plane Super-resolution and Phase Imaging
   10:05Leonhard Möckl (Stanford University, USA)
Quantitative super-resolution microscopy reveals the architecture of the mammalian glycocalyx and its changes during cancer
10:20Coffee break in poster area
11:10 – 12:30Correlative Approaches
Chair: Bernd Rieger
   11:10Rainer Kaufmann (Universität Hamburg, Germany)
Combining SMLM and EM – resolution and structural preservation
   11:35Justin Taraska (NIH, USA)
Imaging the nanoscale structure of the plasma membrane with correlative super-resolution light and electron microscopy
   12:00Silvia Pujals (IBEC & BIST Barcelona, Spain)
Super-Resolution Correlative Light-Electron Microscopy of Bioorthogonal Pathogens: Ultrastructural Imaging of Salmonella-Host Interactions
   12:15Niclas Gimber (Charité Universitätsmedizin Berlin, Germany)
Spatiotemperal analysis of newly exocytosed synaptic vesicle proteins
12:30Closing remarks – outlook to SMLMS2019 in Delft (Bernd Rieger)
13:00Light lunch and departure

 
#SMLMS2018 on Twitter